o:1382
Biološka aktivnost depsidona i atranorina izolovanih iz lišaja Hypogymnia physodes
Biological activity of depsidones and atranorin isolated from lichen Hypogymnia physodes : doctoral dissertation
sr
The objectives of the doctoral dissertation were achieved by determining the chemical composition of the extracts of H. physodes, isolation, structural determination and testing of the biological activity of physodalic acid, physodic acid, 3-hydroxyphysodic acid and atranorin. In this dissertation antioxidant activity, cytotoxic activity on cells of human cervix carcinoma and on rat thymocytes, anticholinesterase activity, antimicrobial activity and influence on the formation of micronuclei in lymphocytes of human blood plasma of physodalic acid, physodic acid, 3-hydroxyphysodic acid and atranorin were investigated The antioxidant activity and anticholinesterase activity were investigated by spectrophotometric methods. Cytotoxic activity on cells of human cervix carcinoma and rat thymocytes was assayed by a colorimetric MTT assay, and CCK-8 assay, respectively. The impact on the formation of micronuclei in lymphocytes of human blood plasma was investigated by micronucleus test. Antimicrobial activity was tested by using microdilution method. Comparing the literature available to us, and these results, it was found that for the first time was investigating the effect of Hypogymnia physodes methanol extract on cells of human cervical cancer and the number of micronuclei in lymphocytes of human blood plasma, impact of atranorin on the number of micronuclei in lymphocytes of human blood plasma and serum activity cholinesterase, antioxidant activity of 3-hydroxyphysodic acid and its effects on rat thymocytes, the number of micronuclei in lymphocytes of human blood plasma, the cells of the human cervical cancer and the activity of serum cholinesterase and the impact physodic acid on rat thymocytes, the number of micronuclei in human lymphocytes blood plasma, the cells of the human cervical cancer and serum cholinesterase activity and antioxidant activity of physodalic acid and its effect on rat thymocytes, the number of micronuclei in lymphocytes of human blood plasma, the cells of the human cervical cancer and the activity of serum cholinesterase.
3-Hydroxyphysodic acid shows a greater anti-oxidant activity from a commercial antioxidant 3,5-di-tert-butyl-4-hydroxytoluene (BHT), and reduce the number of micronucleus cells in human blood plasma
2.2 times more than the commercial protectant amifostine, recommending it as good candidate for in vivo experiments.
Biografija [autora]: listovi [90-93] Datum odbrane: 09.06.2017. Pharmaceutical chemistry
533898134
91552101
5136
91552100
533898134
2017-07-21T10:04:02.053Z
45
no
46
mentor
Igor Ž. 1985-
Stojanović
2017
63
mentor
Andrija
Šmelcerović
2017
63
predsednik komisije
Danica
Agbaba
2017
63
član komisije
Stevo
Najman
2017
63
član komisije
Gordana
Kocić
2017
63
član komisije
Ivana
Arsić
2017
[10], 89, [4] listova
1285000
http://phaidrabg.bg.ac.rs/o:1382
no
yes
4
2017-07-21T10:04:02.320Z
70
Hypogymnia physodes; fizodalična kiselina; fizodična kiselina; 3-hidroksifizodična kiselina; atranorin; antioksidantna aktivnost; citotoksična aktivnost; antiholinesterazna aktivnost; mikronukleusni test; antimikrobna aktivnost
Hypogymnia physodes; physodalic acid; physodic acid; 3-hydroxyphysodic acid; atranorin; antioxidant activity; citotoxic activity; anticholinesterase activity; micronucleus test; antimicrobial activity
577.12:582.282.13(043.3)
B 740
1738
18A05
18A0530
2017