o:1181 Markeri proliferacije i intermedijarnih filamenata u diferencijalnoj dijagnozi benignih i malignih tumora pljuvačnih žlezda Proliferation and intermediate filament markers in the differential diagnosis of benign and malignant tumors of salivary glands : doctoral dissertation sr Introduction: Salivary gland tumors are rather rare neoplasms characterized by a high level of pleomorphism and histological overlapping. Very often, one tumor may contain several cell types, therefore, it is necessary to include additional histochemical and immunohistochemical staining, as well as the morphometric analysis of tumor cells, in terms of an appropriate diagnosis. Materials and methods: The research encompassed 30 benign and 30 malignant tumors, including pleomorphic adenoma (10), Warthin’s tumor (10), basal cell adenoma (6), myoepithelioma (4), adenoid cystic carcinoma (6), mucoepidermoid carcinoma (8), salivary duct carcinoma (6), polymorphous low-grade carcinoma (6), and myoepithelial carcinoma (4). The expression of Ki67, p53, HER-2, p63, CEA, EMA, S-100, CK14, WT-1, GFAP, αSMA and vimentin was analyzed. The morphometric analysis was performed using “ImageJ” software pack. The area, perimeter, Feret diameter, integrated optical density, nucleus circularity and roundness were analyzed. Results: The Ki67 proliferative index was statistically significantly higher in malignant tumors (p<0.001). Adenoid cystic carcinoma exhibited the highest value, whereas the lowest value was exhibited by basal cell adenoma. Immunohistochemically, pleomorphic adenomas were positive to S-100, GFAP, CK14, αSMA, WT1 and EMA. Warthin’s tumor was positive to CK14, CEA and p63; basal cell adenoma showed positivity to S-100, CEA, and vimentin, whereas myoepithelial tumors were positive to vimentin, GFAP, S100, CK14, αSMA. Adenoid cystic carcinoma exhibited positivity to CEA and S-100, mucoepidermoid carcinoma to CK14, salivary duct carcinoma to EMA, CEA, p53 and HER-2, while polymorphous low-grade carcinoma exhibited positivity to CK14, p63, EMA, S100 and vimentin. The morphometric analysis showed statistically significantly increased values of integrated optical density (p<0.001) and nuclear size parameters (p<0,05) in malignant tumors. The highest values for all parameters were exhibited by salivary duct carcinoma Conclusion: The immunohistochemical analysis was used to determine tumor histogenesis with the aim of differentiation. The Ki67 proliferative index is highly useful in the differentiation of benign from malignant tumors. The morphometric parameters of integrated optical density and nuclear size show statistically higher values in the malignant tumor group. Beleška o autoru: list 114 Datum odbrane: 29.08.2016. Pathology 1025596397 91552101 4338 91552100 1025596397 2016-12-14T16:40:51.053Z 45 no 46 mentor Nikola D. 1982- Živković 2016 63 mentor Dragan Mihailović 2016 63 predsednik komisije Maja Jovičić Milentijević 2016 63 član komisije Žaklina Mijović 2016 63 član komisije Zoran Pešić 2016 63 član komisije Svetislav Tatić 2016 114 listova 6485100 http://phaidrabg.bg.ac.rs/o:1181 no yes 4 2016-12-14T16:40:51.320Z 70 Pljuvačne žlezde, benigni tumori, maligni tumori, diferencijalna dijagnoza, imunohistohemija, morfometrija Salivary glands,benign tumors, malignant tumors, differential diagnosis, immunohistochemistry, morphometry 616.316-006.03/.04-079.4-091(043.3) B 520 1738 18A05 18A0518 2016