o:2153 Uticaj tri različite hirurške tehnike na histološke i imunohistohemijske karakteristike venskih graftova Influence of three different surgical techniques on the histological and immunohistochemical characteristics of vein grafts : doctoral dissertation sr Background and Objectives: The saphenous vein is one of the most commonused grafts (SVG) for surgical revascularisation. The mechanism of the SVGsocclusion is still unknown. Surgical preparation techniques have an importantrole in the early and late graft occlusion. Our study analyzed the influence ofthe three different surgical techniques on the histological andimmunohistochemical characteristics of the vein grafts harvested by threedifferent techniques.Methods: Between Jun 2019 and December 2020, 83 patients who underwentsurgical revascularization were prospectively randomly assigned into threegroups according to saphenous vein graft harvesting (conventional (CVH), notouch(NT) and endoscopic (EVH)) technique. The vein graft samples were senton the histological (hematoxylin-eosin staining) and immunohistochemical(CD31, Factor VIII, Caveolin and eNOS) examinations.Results: the CVH, NT, and EVH groups included 27 patients (mean age67.66±5.6), 31 patients (mean age 66.5±7.4) and 25 patients (mean age 66±5.5),respectively. Hematoxylin-eosin staining revealed a lower grade ofmicrostructural vein damage in the NT group (2, IQR 1-2) in comparison withCVH and EVH (3, IQR 2-4), (4, IQR 2-4) respectively (p <0.001).Immunohistochemical examination revealed a high grade of staining in the NTgroup compared to the CVH and EVH group (CD 31 antibody p=0.02,FVIII, p<0.001, Caveolin, p=0.001, and eNOS, p=0.003).The best preservation of the structural vein integrity was in the NT group, whilethe lowest rate of leg wound complication was in the EVH group. Those factsincrease the interest in developing and implementing the endoscopic no-touchtechnique. Bibliografija: listovi 76-84. Datum odbrane: 25.09.2023 Surgery 91552100 126040585 91552101 8647 2024-01-24T13:07:05.053Z 45 no 46 Igor, 1988- Živković 104607753 2023 63 mentor Dragan, 1965- Milić 6320743 2023 63 mentor Slobodan, 1966- Mićović 19192679 2023 63 član komisije Svetozar, 1971- Putnik 12828263 2023 63 član komisije Miljan, 1973- Krstić 4934247 2023 63 član komisije Miloš, 1964- Velinović 12677479 2023 85 listova 2301679 http://phaidrani.ni.ac.rs/o:2153 no yes 12 2024-01-24T13:07:05.320Z 70 Aortokoronarni bypass, Histologija, Imunohistohemija, Klasičnapriprema vene, No-touch, Endoskopska priprema vene CABG, Histology, Immunohistochemistry, Conventional veinharvesting, No-touch, Endoscopic vein harvesting 616.1-089-076:611.018(043.3) B 530 1738 18A05 18A0521 2023