o:2782 Primena histopatoloških promena kao potencijalnih biomarkera u akvatičnoj ekotoksikologiji za procenu toksičnog efekta nanočestica na model organizmu Chironomus riparius Aplication of histopathological changes as potential biomarkers in aquatic ecotoxicology assessment of nanoparticle toxic effect on model organism Chironomus riparius : doctoral dissertation sr The aquatic environment is the largest collector of waste, which enters it directly, through dumping and releasing waste, or indirectly, through soil runoff, and wind deposition. This implies the need for detailed research that would contribute to a better understanding of individual pollutant toxicity as well as designing the best mechanisms for risk assessment and defining guidelines and laws on waste management.In this dissertation, Chironomus riparius was used as a model organism, with histological analysis initially used to describe the normal morphology of the digestive and excretory systems, as well as adipose tissue.A multi-biomarker approach was used for detecting the toxicity of metal-oxide nanoparticles (TiO2, CeO2, Fe3O4), LCFA, and MP. First, the characterization of the tested particles was done in order to get more information about them. Metal-oxide nanoparticles and MP were described in previous studies, so the original characterization of LCFA particles was done in the dissertation.An acute test with metal-oxide nanoparticles revealed the high toxicity of nano-Fe3O4 that caused intensive vacuolization of the midgut region I and Malphigian tubules. Nano-CeO2 caused mild vacuolization of Malphigian tubules. Nano-TiO2 provoked the remodeling of fat body tissue and gut epithelium. By analyzing sub-organismal biomarkers in chronic tests, it was revealed that metal oxide nanoparticles and MP have the potential to affect oxidative stress enzymes, while nano-CeO2 and nano-Fe3O4 have also been shown to be genotoxic. LCFA particles affected almost all analyzed parameters.Microvilli length has been shown to be a parameter that has a strong potential to become a histopathological biomarker that can be used in ecotoxicological studies in the future. Biografija autora sa bibliografijom: listovi 154-158.Bibliografija: listovi 99-136. Datum odbrane: 29.8.2024. Cell and tissue biology and ecotoxicology 91552100 159498505 91552101 8724 2024-12-24T09:50:24.053Z 45 no 46 Jelena S. Stojanović 102529801 2024 63 mentor Đurađ, 1983- Milošević 14138471 2024 63 član komisije Aleksandra, 1975- Zarubica 11093863 2024 63 član komisije Jelena, 1982- Rajković 8468839 2024 63 član komisije Margareta, 1982- Kračun Kolarević 23982439 2024 63 član komisije Dimitrija, 1990- Savić-Zdravković 59660553 2024 [16], 159 listova 8369833 http://phaidrani.ni.ac.rs/o:2782 no yes 12 2024-12-24T09:50:24.320Z 70 Chironomus riparius, histology, biomarkers, ecotoxicology, nanoparticles, microplastic, metal oxides, genotoxicity, oxidative stress, hemoglobin, OECD protocols, microvilli Chironomus riparius, histologija, biomarkeri, ekotoksikologija, nanočestice, mikroplastika, oksidi metala, genotoksičnost, oksidativni stres, hemoglobin, OECD protokoli, mikrovili [616-091.8+615.9]:597.7(043.3) B 003B 210 1738 18A07 18A0704 2024