o:3282 Extracellular vesicles as biomarkers of disease activity in antineutrophil cytoplasmic antibody-associated vasculitis: doctoral dissertation Ekstraćelijske vezikule kao biomarkeri aktivnosti bolesti kod vaskulitisa udruženim sa antitelima protiv citoplazme neutrofila : doctoral dissertation en The purpose of this scientific investigation was to evaluate the degree ofinflammation and disease activity using activity markers on extracellularvesicles in patients with ANCA-positive vasculitis, and to compare findingswith healthy controls. The research was conducted in collaboration withKarolinska University Hospital, Solna, Sweden, within ERASMUS plusexchange program between University of Niš and Karolinska Institute. Data andbiological samples were obtained from the VASKA cohort, which includedpatients with active (defined as BVAS≥1, infection excluded) and inactiveANCA vasculitis, i.e. in remission (BVAS=0). Age- and sex-matched healthyindividuals served as control subjects. Concentration and phenotype ofextracellular vesicles in plasma were assessed by flow cytometry, in both activeand inactive disease, and compared to healthy controls. In addition, the procoagulantproperties of extracellular vesicles were examined through their effecton thrombin formation, using a modified method based on CalibratedAutomated Thrombogram (CAT) assay. The results demonstrated significantlyelevated levels of extracellular vesicles bearing PTX3, HMGB1, TWEAK andcomponents of complement system (C3a and C5a) in patients with active diseasecompared to those in remission and with healthy subjects. This studydemonstrates that circulating extracellular vesicles are significantly elevated inactive AAV and correlate with clinical disease activity (BVAS). These findingssupport their potential role as biomarkers for disease monitoring. References: list.101-123. Biography: list.124. Datum odbrane: 17.12.2025. Pediatrics, Immunology, Rheumatology 91552100 191069193 91552101 8834 2026-05-12T14:20:17.053Z 45 no 46 Milena, Manojlović 73818633 2025 63 član komisije Miloš, 1982- Kostić 4731751 2025 63 mentor Jelena, 1965- Vojinović 10749543 2025 63 član komisije Aleksandra Antović 68812297 2025 127 list. 6637337 http://phaidrani.ni.ac.rs/o:3282 no yes 12 2026-05-12T14:20:17.320Z 70 extracellular vesicles, ANCA associated vasculitis, biomarkers, diseaseactivity eкстрацелуларне везикуле, ANCA асоцирани васкулитис, биомаркери, активност болести 612.017:616-053.2(043.3) B 007;B 660;B 500;B 580 1738 18A05 18A0526 2025